Abstract: Strict requirements for climatic-ecological conditions are needed for the two-line hybrid rice cultivation. While the seed production bases are concerning, full diagnosis and scientific assessment should be conducted on the local climatic-ecological adaptability. The special influences of climatic-ecological conditions on two-line hybrid rice are analyzed. It is pointed out that the two safety periods should be considered in seed production. A diagnosis method for analyzing the periods is given. The “Hunan Provincial Expert Service System for Climate Diagnose and Policy-making on Seed Production of Two-line Hybrid Rice” is developed and the system can be used to define the available zones and time-sections in Hunan for seed production. In the beginning of 2000, this proposal used by the system was adopted in Hunan Province and the management of the seed production is further promoted.