Abstract: Global change and regional response are focused on by ecologists, botanist, geographers and agronomist. Rising temperature, precipitation distribution and land use pattern changes are caused by global change. The study on response and adaptation of grassland to CO₂ enrichment and climate change is the key aspect that relationship between terrestrial ecosystem and global change is understood and predicted. This paper reviewed the effects of rising temperature, CO₂ enrichment and water status change on grassland ecosystem recent several years, and put forward on 8 key scientific issues. It is believed that this paper will be useful to deeply understand the response of grassland ecosystem to global change and to discovery the methods that weaken the negative effects of global change, inspire mind, simulate interest, and provide the study basis.