Li Hualong, Wang Jinghong, Zhang Weimin, et al. Effects of high temperature stress on leaf chlorophyll fluorescence characteristics of kiwifruit. J Appl Meteor Sci, 2021, 32(4): 468-478. DOI:  10.11898/1001-7313.20210408.
Citation: Li Hualong, Wang Jinghong, Zhang Weimin, et al. Effects of high temperature stress on leaf chlorophyll fluorescence characteristics of kiwifruit. J Appl Meteor Sci, 2021, 32(4): 468-478. DOI:  10.11898/1001-7313.20210408.

Effects of High Temperature Stress on Leaf Chlorophyll Fluorescence Characteristics of Kiwifruit

DOI: 10.11898/1001-7313.20210408
  • Received Date: 2021-04-06
  • Rev Recd Date: 2021-06-09
  • Publish Date: 2021-07-31
  • Kiwifruit is a vine with poor resistance to high temperature. The original habitat is mostly semi shade environment under mountain forest, with humid air, mild temperature change and weak light. The main problem in production is that the temperature of tree is often too high when the tree is introduced from the original forest environment in mountainous areas to cultivated under the direct sunlight in farmland. The leaves, fruits and trunks often get damaged.With the background of climate warming, in Shaanxi, the main kiwifruit producing area, extreme high temperature weather with daily maximum temperature over 40 ℃ often occurs. The high temperature damage of kiwifruit is particularly prominent, such as leaf wilting, shedding, fruit sunburn, fruit drop, and even tree death.In order to explore the effects of high temperature stress on photosynthetic apparatus of kiwifruit leaves and establish a heat injury identification index based on chlorophyll fluorescence response, the variation characteristics of the FO(minimal recorded fluorescence intensity), Fm(maximal recorded fluorescence intensity), Fa(maximal photochemistry efficiency), ΔWK(relative variable fluorescence difference at 300 μs), Tr(trapped energy flux per area at t=0), Et(electron transport flux per area at t=0), Dd(dissipated energy flux per area at t=0), Rm (density of QA-reducing PSⅡ reaction centers) in kiwifruit leaves under 30 ℃, 33 ℃, 36 ℃, 39 ℃, 42 ℃, 45 ℃, 48 ℃, 50 ℃, 52 ℃, 54 ℃ condition are studied by using the technique of fast chlorophyll fluorescence induction dynamics analysis(JIP-test). The results show that Tr, Rm and ΔWK are all affected by temperature stress in the range of 30-54 ℃, which belongs to PSⅡ sensitive site parameters, in which Tr, Rm show a linear downward trend with the increase of stress temperature, while ΔWK shows an exponential upward trend with the increase of stress temperature. FO, Fm, Fa, Dd, Et show stable or less variable under lower temperature stress, and intensified under higher temperature stress, which belongs to the secondary sensitive site parameters of PSⅡ. Most chlorophyll fluorescence parameters have two mutation critical points at 39 ℃ and 45 ℃. The results show that kiwifruit leaves have mild temperature stress at 30 ℃ ≤ T < 39 ℃, moderate temperature stress at 39 ℃ ≤ T < 45 ℃, and severe temperature stress at T ≥ 45 ℃.
  • Fig. 1  The fast chlorophyll fluorescence induction dynamics curve of kiwifruit leaves under different high temperature stress conditions

    Fig. 2  Effects of high temperature stress on the relative variable fluorescence (ΔVt) for kiwifruit leaves

    VK, ΔVJ and ΔVI represent the relative variable fluorescence at t=300 μs, 2 ms, 30 ms, respectively)

    Fig. 3  Effects of high temperature stress on the relative variable fluorescence Wt and ΔWt for kiwifruit leaves

    Fig. 4  Effects of high temperature stress on FO of kiwifruit leaves

    Fig. 5  Effects of high temperature stress on Fm of kiwifruit leaves

    Fig. 6  Effects of high temperature stress on Fa of kiwifruit leaves

    Fig. 7  Effects of high temperature stress on Tr, Et, Dd, Rm of kiwifruit leaves

    Table  1  Formulae and terms used in the analysis of OJIP fluorescence induction dynamics curve

    术语和公式 定义
    FO 暗适应后20 μs时的荧光强度
    FK K相处(300 μs)的荧光强度
    FI I相处(2 ms)的荧光强度
    FJ J相处(30 ms)的荧光强度
    FP 最大荧光处(P相)的荧光强度
    Fm=FP 暗适应后的最大荧光强度
    Fv=Fm-FO t时刻的可变荧光强度
    Vt=(Ft-FO)/(Fm-FO) t时刻的相对可变荧光强度
    VI=(FI-FO)/(Fm-FO) I相的相对可变荧光强度
    VJ=(FJ-FO)/(Fm-FO) J相的相对可变荧光强度
    MO=4×(FK-FO)/(Fm-FO) OJIP荧光诱导曲线的初始斜率
    φP=1-(FO/Fm) PSⅡ最大光化学效率
    φE=(1-(FO/Fm))×ψO 用于电子传递的量子产额
    ψO=(1-VJ) 将电子传递到初级醌受体以后其他电子受体的概率
    Fa=Fv/Fm 暗适应下PSⅡ的最大量子产额
    AcFO 单位面积吸收的光能
    Rm=φP×(VJ/MOAc 单位面积有活性的反应中心数量
    Tr=φP×Ac 单位面积捕获的光能
    Et=φE×Ac 单位面积用于电子传递的能量
    Dd=Ac-Tr 单位面积的热耗散
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  • [1]
    Huang H W. History of 100 years of domestication and improvement of kiwifruit and gene discovery from genetic introgressed populations in the wild. Chinese Bulletin of Botany, 2009, 44(2): 127-142. doi:  10.3969/j.issn.1674-3466.2009.02.001
    Wang Z, Luo H, Li Y L, et al. Effects of urbanization on temperatures over the Qinling Mountains in the past 50 years. Journal of Applied Meteorological Science, 2016, 27(1): 85-94. doi:  10.11898/1001-7313.20160109
    Chen Y, Ren G Y, Wang L, et al. Temporal change of warm winter events over the last 56 years in China. Journal of Applied Meteorological Science, 2009, 20(5): 539-545. doi:  10.3969/j.issn.1001-7313.2009.05.004
    Zhao P, Nan S L. Some advances in climate and climate change research. Journal of Applied Meteorological Science, 2006, 17(6): 725-735. doi:  10.3969/j.issn.1001-7313.2006.06.010
    Li X M, Bai Q F, Zhu L. The Influence of climate change on suitability of Shaanxi apple growth. Journal of Applied Meteorological Science, 2011, 22(2): 241-248. doi:  10.3969/j.issn.1001-7313.2011.02.013
    Lü Y, Guo Y S. Analysis on the cause of kiwifruit sunburn caused by scattered light. Northwest Horticulture, 1999(3): 20-22.
    Shi C H, Wang X Q, Luo J. Antioxidant physiological response and threshold temperature of sunburn injury in kiwifruit at high temperature. Acta Agriculturae Shanghai, 2017, 33(4): 72-76.
    Li X H, Pan X H, Li X. Effects of high temperature and drought on growth and development of kiwifruit and countermeasures. Northwest Horticulture, 2017(5): 24-26.
    Toth Z Z, Schansker G, Gara G, et al. Photosynthetic electron transport activity in heat-treated barley leaves: The role of internal alternative electron donors to photosystemⅡ. Biochimica et Biophysica Acta, 2007, 767(4): 295-305.
    Jiang C D, Gao H Y, Zou Q. Leaf orientation, photorespiration and xanthophyll cycle protect young soybean leaves against high irradince in field. Environmental and Experimental Botany, 2006, 55: 87-96. doi:  10.1016/j.envexpbot.2004.10.003
    van Heerden P D R, Swanepoel J W, Krüger G H J. Modulation of photosynthesis by drought in two desert scrub species exhibiting C3-mode CO2 assimilation. Environmental and Experimental Botany, 2007, 61(2): 124-136. doi:  10.1016/j.envexpbot.2007.05.005
    Georgieva K, Tsonev T, Velikova V, et al. Photosynthetic activity during high temperature of pea plants. Journal of Plant Physiology, 2000, 157(2): 169-176. doi:  10.1016/S0176-1617(00)80187-X
    Wang Z X, Ai J, Chen L, et al. Activity of photosystemsⅡin leaves of actinidia arguta under different temperature treatments. Acta Botanica Boreali-Occidentalia Sinica, 2015, 35(2): 329-334.
    Zhong M, Zhang W B, Zou L F, et al. Diurnal variation of photosynthesis and chlorophyll fluorescence characteristics in kiwifruit under high temperature condition. Acta Agriculturae Universitatis Jiangxiensis, 2018, 40(3): 472-478.
    Du G D, Lü D G, Zhao L, et al. Effects of high temperature on leaf photosynthetic characteristics and photosystemⅡ photochemical activity of kernel-used apricot. Chinese Journal of Applied Ecology, 2011, 22(3): 701-706.
    Chen J J, Li L C, Lin J, et al. Integrated risk evaluation on meteorological disasters of loquat in Fujian Province. Journal of Applied Meteorological Science, 2014, 25(2): 232-241. doi:  10.3969/j.issn.1001-7313.2014.02.013
    Yang K, Chen B B, Chen H, et al. Comprehensive climatic index and grade classification of cold damage for Taiwan green jujube in Fujian. Journal of Applied Meteorological Science, 2020, 31(4): 427-434. doi:  10.11898/1001-7313.20200405
    Li P M, Gao H Y, Strasser R J. Application of chlorophyll fluorescence dynamics to the study of phytobiology. Journal of Plant Physiology and Molecular Biology, 2005, 31(6): 559-566.
    Strasser B J. Donor side capacity of photosystemⅡprobed by chlorophyll fluorescence transients. Photosynthesis Research, 1997, 52(2): 147-155.
    Strasser R J, Srivastava A, Tsimilli-Michael M. The Fluorescence Transient as a Tool to Characterise and Screen Photosynthetic Samples. Bristol: Taylor and Francis, 2000: 445-483.
    Oukarroum A, Schansker G, Strasser R J. Drought stress effects on photosystem I content and photosystemⅡ thermotolerance analyzed using Chl a fluorescence kinetics in barley varieties differing in their drought tolerance. Physiologia Plantarum, 2009, 137(2): 188-199.
    Guo J P. Research progress on agricultural meteorological disaster monitoring and forecasting. Journal of Applied Meteorological Science, 2016, 27(5): 620-630. doi:  10.11898/1001-7313.20160510
    Qu Z J, Zhou G S, Wei Q P. Meteorological disaster index and risk assessment of frost injury during apple florescence. Journal of Applied Meteorological Science, 2016, 27(4): 385-395. doi:  10.11898/1001-7313.20160401
    Lu K D, Huang W H, Fang L, et al. The climatic zoning of spring maize in Hunan based on meteorological disaster indexes. Journal of Applied Meteorological Science, 2007, 18(4): 548-553.
    Zhong C H. Advances in Actinidia Research(Ⅸ). Beijing: Science & Technology Press, 2019.
    Yuan J L, Ma C, Feng Y L, et al. Response of chlorophyll fluorescence transient in leaves of wheats with different drought resistances to drought stresses and rehydration. Plant Physiology Journal, 2018, 54(6): 1119-1129.
    Lu S Y, Yang Z Q, Zhang Y D, et al. Effect of photoperiod on fluorescence characteristics of photosynthetic system of fresh-cut chrysanthemum leaves under high temperature. Chinese Journal of Agrometeorology, 2020, 41(10): 632-643.
    Chen B, Zhang J, Ma X H, et al. Influences of exogenous selenium on the chlorophyll fluorescence characteristics and chemical composition in flue-cured tobacco under drought stress. Journal of Agricultural Science and Technology, 2018, 20(10): 95-104.
    Li P, Cheng L, Gao H, et al. Heterogeneous behavior of PSⅡin soybean(Glycine max) leaves with identical PSⅡ photochemistry efficiency under different high temperature treatments. Journal of Plant Physiology, 2009, 166(15): 1607-1615.
    Jin L Q, Che X K, Zhang Z S, et al. The relationship between the changes in Wk and different damage degree of PSⅡ donor side and acceptor side under high temperature with high light in cucumber. Plant Physiology Journal, 2015, 51(6): 969-976.
    Chen Y Z, Li X P, Xia L, et al. The application of chlorophyll fluorescence technique in the study of responses of plants to environmental stresses. Journal of Tropical and Subtropical Botany, 1995, 3(4): 79-86.
    Bilger H W, Schreiber U, Lange O L. Determination of leafheat resistance: Comparative investigation of chlorophyll fluorescence changes and tissue necrosis methods. Oecologia, 1984, 63(2): 256-262.
    Schreiber U, Berry J A. Heat induced changes in chlorophyll fluorescence in intact leaves correlated with damage of the photosynthetic apparatus. Planta, 1977, 136: 233-238.
    Seemann J R, Downton W J, Berry J A. Temperature and leaf osmotic potential as factors in the acclimation of photosynthesis to high temperature in desert plants. Plant Physiology, 1986, 80: 926-930.
    Feng J C, Hu X L, Mao X J, et al. Application of chlorophyll fluorescence dynamics to plant physiology in adverse circumstance. Economic Forest Researche, 2002, 20(4): 14-18.
    Li X, Feng W, Zeng X C. Advances in chlorophyll fluorescence analysis and its uses. Acta Botanica Boreali-Occidentalia Sinica, 2006, 26(10): 2186-2196.
    Yamane Y, Kashino Y, Koike H, et al. Increases in the fluorescence level and Fo level and reversible inhibition of photosystem Ⅱreaction center by high-temperature treatment in higher plants. Photosynthesis Research, 1997, 52: 57-64.
    Srivastava A, Strasser R.J. Stress and stress management of land plants during a regular day. Journal of Plant Physiology, 1996, 148: 445-455.
    Yamane Y, Kashino Y, Koike H. Effects of high temperatures on the photosynthetic systems in spinach: Oxygen evolving activities, fluorescence characteristics and the denaturation process. Photosynthesis Res, 1998, 57: 51-59. doi:  10.1023/A:1006019102619
    Wang M, Gao Z K, Huang R H, et al. Heat stress characteristics of photosystemⅡin eggplant. Chinese Journal of Applied Ecology, 2007, 18(1): 63-68.
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