Abstract: This paper mainly discusses the experimental results of the suppression effect on methane gas (CH4) released from the paddy field by the Effective Microorganism (EM), and the results show that the suppression effect was more remarkable, averagely more than 59% being decreased in the first year. Moreover, the frequency of negative flux which represents the paddy field as the sink to absorb CH4 from atmosphere increased. It may result from the action of photosynthetic bacteria contained in EM. Furthermore, the experiment revealed that the EM could get a raise in yield of crop without chemical fertilizer.