Effects of High Temperature Stress on Leaf Chlorophyll Fluorescence Characteristics of Kiwifruit

Li Hualong; Wang Jinghong; Zhang Weimin; Bai Qinfeng; Zhang Tao; Pan Yuying; Quan Wenting

doi:10.11898/1001-7313.20210408

Vol.32, NO.4, 2021

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Article Navigation > Journal of Applied Meteorological Science > 2021 > 32(4): 468-478

Li Hualong, Wang Jinghong, Zhang Weimin, et al. Effects of high temperature stress on leaf chlorophyll fluorescence characteristics of kiwifruit. J Appl Meteor Sci, 2021, 32(4): 468-478. DOI: 10.11898/1001-7313.20210408.

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Li Hualong, Wang Jinghong, Zhang Weimin, et al. Effects of high temperature stress on leaf chlorophyll fluorescence characteristics of kiwifruit. J Appl Meteor Sci, 2021, 32(4): 468-478. DOI: 10.11898/1001-7313.20210408.

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Effects of High Temperature Stress on Leaf Chlorophyll Fluorescence Characteristics of Kiwifruit

DOI: 10.11898/1001-7313.20210408

Shaanxi Meteorological Service Center of Agricultural Remote Sensing and Economic Crop, Xi'an 710015

Received Date: 2021-04-06
Rev Recd Date: 2021-06-09
Publish Date: 2021-07-31

Abstract

Abstract

Kiwifruit is a vine with poor resistance to high temperature. The original habitat is mostly semi shade environment under mountain forest, with humid air, mild temperature change and weak light. The main problem in production is that the temperature of tree is often too high when the tree is introduced from the original forest environment in mountainous areas to cultivated under the direct sunlight in farmland. The leaves, fruits and trunks often get damaged.With the background of climate warming, in Shaanxi, the main kiwifruit producing area, extreme high temperature weather with daily maximum temperature over 40 ℃ often occurs. The high temperature damage of kiwifruit is particularly prominent, such as leaf wilting, shedding, fruit sunburn, fruit drop, and even tree death.In order to explore the effects of high temperature stress on photosynthetic apparatus of kiwifruit leaves and establish a heat injury identification index based on chlorophyll fluorescence response, the variation characteristics of the F_O(minimal recorded fluorescence intensity), F_m(maximal recorded fluorescence intensity), F_a(maximal photochemistry efficiency), ΔW_K(relative variable fluorescence difference at 300 μs), T_r(trapped energy flux per area at t=0), E_t(electron transport flux per area at t=0), D_d(dissipated energy flux per area at t=0), R_m (density of QA-reducing PSⅡ reaction centers) in kiwifruit leaves under 30 ℃, 33 ℃, 36 ℃, 39 ℃, 42 ℃, 45 ℃, 48 ℃, 50 ℃, 52 ℃, 54 ℃ condition are studied by using the technique of fast chlorophyll fluorescence induction dynamics analysis(JIP-test). The results show that T_r, R_m and ΔW_K are all affected by temperature stress in the range of 30-54 ℃, which belongs to PSⅡ sensitive site parameters, in which T_r, R_m show a linear downward trend with the increase of stress temperature, while ΔW_K shows an exponential upward trend with the increase of stress temperature. F_O, F_m, F_a, D_d, E_t show stable or less variable under lower temperature stress, and intensified under higher temperature stress, which belongs to the secondary sensitive site parameters of PSⅡ. Most chlorophyll fluorescence parameters have two mutation critical points at 39 ℃ and 45 ℃. The results show that kiwifruit leaves have mild temperature stress at 30 ℃ ≤ T < 39 ℃, moderate temperature stress at 39 ℃ ≤ T < 45 ℃, and severe temperature stress at T ≥ 45 ℃.
- kiwifruit;
- temperature stress;
- chlorophyll fluorescence parameters

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Figures and Tables

Fig. 1 The fast chlorophyll fluorescence induction dynamics curve of kiwifruit leaves under different high temperature stress conditions

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图 2 高温胁迫对猕猴桃叶片ΔV_t影响

(ΔV_K，ΔV_J和ΔV_I为分别在300 μs，2 ms和30 ms处测定的ΔV_t)

Fig. 2 Effects of high temperature stress on the relative variable fluorescence (ΔV_t) for kiwifruit leaves

(ΔV_K, ΔV_J and ΔV_I represent the relative variable fluorescence at t=300 μs, 2 ms, 30 ms, respectively)

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Fig. 3 Effects of high temperature stress on the relative variable fluorescence W_t and ΔW_t for kiwifruit leaves

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Fig. 4 Effects of high temperature stress on F_O of kiwifruit leaves

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Fig. 5 Effects of high temperature stress on F_m of kiwifruit leaves

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Fig. 6 Effects of high temperature stress on F_a of kiwifruit leaves

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Fig. 7 Effects of high temperature stress on T_r, E_t, D_d, R_m of kiwifruit leaves

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Table 1 Formulae and terms used in the analysis of OJIP fluorescence induction dynamics curve

术语和公式	定义
F_O	暗适应后20 μs时的荧光强度
F_K	K相处(300 μs)的荧光强度
F_I	I相处(2 ms)的荧光强度
F_J	J相处(30 ms)的荧光强度
F_P	最大荧光处(P相)的荧光强度
F_m=F_P	暗适应后的最大荧光强度
F_v=F_m-F_O	在t时刻的可变荧光强度
V_t=(F_t-F_O)/(F_m-F_O)	在t时刻的相对可变荧光强度
V_I=(F_I-F_O)/(F_m-F_O)	I相的相对可变荧光强度
V_J=(F_J-F_O)/(F_m-F_O)	J相的相对可变荧光强度
M_O=4×(F_K-F_O)/(F_m-F_O)	OJIP荧光诱导曲线的初始斜率
φ_P=1-(F_O/F_m)	PSⅡ最大光化学效率
φ_E=(1-(F_O/F_m))×ψ_O	用于电子传递的量子产额
ψ_O=(1-V_J)	将电子传递到初级醌受体以后其他电子受体的概率
F_a=F_v/F_m	暗适应下PSⅡ的最大量子产额
A_c≈F_O	单位面积吸收的光能
R_m=φ_P×(V_J/M_O)×A_c	单位面积有活性的反应中心数量
T_r=φ_P×A_c	单位面积捕获的光能
E_t=φ_E×A_c	单位面积用于电子传递的能量
D_d=A_c-T_r	单位面积的热耗散

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